Queen Street Mill is a Grade I listed building in Harle Syke, a suburb to the north-east of Burnley, Lancashire. It was built in 1894 for the Queen Street Manufacturing Company. It closed on 12 March 1982 and was mothballed, but was subsequently taken over by Burnley Borough Council and maintained as a museum.

In the 1990s ownership passed to Lancashire Museums. Unique in being the world’s only surviving operational steam-driven weaving shed, it received an Engineering Heritage Award in November 2010.